Characterization of cancer stem cell (CSC) markers provides potential targets for CSC-direct therapy. However, the CSC phenotype varies substantially among patients. Therefore, fast and accurate identification of personalized CSC surface markers is critical determining the precision of CSC-specific targeting. Mid-to high-throughput profiling real-time PCR can simultaneously quantify the gene expression panel of hundreds of CSC surface markers and CSC specially-activated signal pathway molecules with minimal samples. Through detecting CSC markers of circulating tumor cells, the PCR assay can lead to early diagnosis of tumors, evaluation of tumor treatment effects, and monitoring of tumor recurrence. Together, mid-to high-throughput profiling real-time PCR assays may generate high-quality data, providing over millions of pre-designed primer/probes sets for a specific biological process, covering multiple species, which is promising and may find valuable usages in both basic research and clinical trials.
Cancer stem cells, Markers, Mid-to high- throughput profiling real-time PCR, Cancer immunotherapy.