Dr. Richard Obiso

• Obiso , R. Antadze, I. , Gunia S, Balarjishvili N, a nd Kutateladze. The bacteriophage of Francisella tularensis . Bacteriophage. In review. 2018.

• M. Roena Sukhiashvili, Ekaterine Zhgenti, Ekaterine Khmaladze, Irma Burjanadze, Paata Imnadze, Ju Jiang , Heidi St. John, Christina Farris, Theresa Gallagher, O biso , Allen L. Richards . 2017 . Identification, Distribution, and Prevalence of Tick - borne Spotted Fever Group Rickettsiae in Georgia . Tick and Tick - borne Diseases. 201 8 In press

• K. Sidamonidze, W. Su, E. Zhgenti, F. Buyuk, M. Sahin, N. Trapaidze, P. I mnadze, M. P. Nikolich, R. Obiso , and A. Kotorashvili. 2017. Molecular typing of Brucella species strains from Georgia and Northeastern Turkey. Journal of Bacteriology and Mycology 4(3) 1 - 8.

• Khmaladze E, Su W, Zghenti E, Buyuk F, Sahin M, Nicolich MP, Baillie L, Obiso R and Kotorashvili A . 2017. Molecular typing of Bacillus anthracis species strains from Georgia and Turkey. Journal of Bacteriology and Mycology 4(3 ) 1 - 8.

• Antadze I, Dadunashvili M, Burbutashvili T, Gunia S, Balarjishvili N, Tevdoradze E, Pataridze T, Obiso R, Hagius S, Elzer P and Kutateladze M. 2017 . Diversity of Phage - Host Specificity in Brucella Phage. Journal of Bacteriology and Mycology. 4(2): 1 - 8.

• Marco De Nardi , Anaïs Léger , Ta tul Stepanyan , Bagrat Khachatryan , Talgat Karibayev , Igor Sytnik , Samat Tyulegenov , Assel Akhmetova , Se rhiy Nychyk , Mykola Sytiuk , Oleg Nevolko , Roman Datsenko , Tengiz Chaligava , Lasha Avaliani , Otar Parkadze , Lena Nini dze , Natia Kartskhia , Tsira Napetvaridze , Zviad Asanishvili , Demna Khelaia , Ioseb Menteshashvili , Meruzhan Zad ayan , Lyudmila Niazyan , Nataliya Mykhaylovska , Bradford Raymond Brooks , Gulnara Zhumabayeva , Saltanat Satabayeva , Magda Metreveli , Theresa Gallagher, and Richard Obiso . 2017. Implementation of a Regional Training Program on African Swin e Fever as Part of the Cooperative Biological Engagement Program across the Caucasus Region. Frontiers in Veterinary Medicine , Curriculum, Instruction, and Pedagogy. V ol 4, article 164 (1 - 11 ) .

• Nozadze M, Zhgenti E, Meparishvili M, Tsverava L, Kiguradze T, Chanturia G, Babuadze G, Kekelidze M, Bakanidze L , Shutkova T, Imnadze P, Francesconi SC, Obiso R, Solomonia R. 2015.

Comparative Proteomic Studies of Yersinia pestis Strains Isolated from Natural Foci in the Republic of Georgia. Front Public Health . 2015 Oct 16; 3:239 .

• Obiso, R ., 2012. Georgian Inspiration at Attimo (Whitebarrel) . Virginia Wine Guide Online.

• Zh genti, E., N. Trapaidze, K. Sidamonidze, M. Zakalashvili, M. Ramishvili, L. Malania, M. Grdzelidze, T. Akhvlediani, I. Kokaia , L. Sanodze, T. Onashvili, E. Mamisahsvili, M. Nikolich, R. Obiso , R. Rivard, P. Elzer. 2011. “Multiple - locus variable - number tandem repeats analysis (MLVA) - based genetic diversity of human and animal Brucella isolates from Georgia ” American Society for Tropical Medic ine and Hygiene Annual Meeting, Poster LB - 2138. December 2011.

• Obiso , R., “Strengthening Global Health through Capacity Building under the Cooperative Biological Engagement Program”. 2011, Ses sion Chair. Chemical and Biological Defense Science and Te chnology Conference, DTRA, Las Vegas, NV, November 2011.

• Obiso , R., “Animal Health Research Projects in Georgia”. 2011. Chemical and Biological Defense Science and Technology Conference , DTRA, Las Vegas, NV, November 2011.

• Obiso , R., “Capacity Building and Establishing Sustainable Research Programs in Georgia”. 2011.

Chemical and Biological Defense Science and Technology Conference, DTRA, Las Vegas, NV, November 2011.

• Janelidze N, Jaiani E, Lashkhi N, Tskhvediani A, Kokashvili T, Gvarishvili T, Jgenti D, Mikashavidze E, Diasamidze R, Narodny S, Obiso R, Whitehouse CA, Huq A, Tediashvili M. 2011 . Microbial water quality of the Georgian coastal zone of the black sea. Mar Pollut Bull. Mar;62(3):573 - 80

• Zhgenti, G. Chanturia, R. Jashi, M. Kekelidze, R . Obiso, S.C. Francesconi, L. Bakanidze, N. Tsertsvadze. 2010 . Rapid Identification of Atypical Y. pestis Strains Isolated in Natural Foci of Georgia. Yersinia 2010: 10 th symposium on Yersinia, October 2010, Recife, Brazil.

• Antadze, T. Burbutashvili, S. Gunia, M. Dadunashvili, N. Balarjishvili, T. Patarize, P. Elzer, R. Obiso , M. Kutateladze. 2010 . Antibiotic - and phage susceptibility of Brucella bacterial strains from the Eliava Institute collection . American Society for Microbiology, 2nd ASM Confer ence on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the Environment . June 2010, Toronto Canada.

• Kutateladze, I. Antadze, T. Burbutashvili, S. Gunia, M. Dadunashvili, N. Balarjishvili, T. Pataridze, and R. O biso . 2010. Characterization of bacteriophages against Brucella species . Viruses of Microbes Conference. Institute Pasteur, Paris, France.

• Imnadze, P., N. Tsertsvadza, L. Bakanidze, S. Tsanava, Z. Safarova, N. Bamabishvili, T. Onashvili, R. Obiso , and M. Zakareshvili. 2010 . Ecology, Genetic Clustering, and Virulence of Major Bacterial and Viral Pathogens in the Republic of Georgia , ASM Biodefense Annual Meeting, Baltimore, MD

• Imnadze, P., S. Tsanava, G. Katsitadze, L. Malania, N. Tsertsvadze, S. Fra ncesconi, R. Obiso , and L. Bakanidze. 2009 . B. anthracis Ecology and Infection in Georgia. Bacillus ACT Meeting, Santa Fe, NM

• MD Akhedova, OS Kasimov, UN Imomaliev, AV Khodiev, MJ Nikolich, DV Clarke, R. Obiso , and PH Elzer. 2009 . Brucellosis in Endemi c Regions of Uzbekistan. 2009.

• Onashvili, T., M. Nikolaishvili, M. Zakareshvili, I. Beradze, M. Donduashvili, M. Kokhredize, T. Tighilauri, K. Goginashvili, G. Osiashvili, R. Obiso , P. Elzer. 2009 . Clinical, Epidemiological, and Laboratory based asses sment of Brucellosis in Georgia (poster presentation). CRAWD Brucellosis section meeting and conference. Chicago, Il.

• Kutateladze, M., R. Obiso , I. Antadze, T. Burbutashvili, S. Gunia, M. Dadunashvili, N. Balarjishvili, and T. Pataridze. 2009 . Characte rization and Hose Dependent modulation of bacteriophage in Brucella species . (poster presentation). CRAWD Conference of Research Workers of Animal Disease Annual Meeting, Chicago, Il.

• Belcher, N.B. and R.J. Obiso , 2007 . Bacteriophage uses to decontam inate food (poster presentation) . ISOPOL XVI: 16th International Symposium on Problems of Listeriosis , Savannah, Georgia.

• Obiso, R ., and A. Lorincz. 2005/2006 . Digene Corporation VIP lecture series, “Specimen Preparation System”. 6 lectures on clinica l specimen preparation, processing, and automation. Gaithersburg, MD

• Obiso, R ., and A. Lorincz. 2004 . Digene Corporation Company Profile. Pharmacogenomics. 5(1):129 - 32 .

• Obiso, R.J., Jr., 2002 . Microbial Genomics and novel antibiotics. Drug Discove ry and Development. 2(6):6.

• Obiso, R.J., Jr., 2002 . A novel approach to detecting gene expression. Genomics and Proteomics. 2(3):46 - 47.

• Obiso, R.J ., S. Bingham, C. Strange, J. Zakel, J. Hernandez, D. Seyfried, and J. Lazar. 2002 . The HC Express Arra y Kit can be used in conjunction with RNA amplification protocols to detect RNA from limiting sources (poster presentation). Biomolecular Technologies: Tools for Discovery in Proteomics and Genomics . Austin, TX.

• Obiso, R.J., S. Bingham, C. Strange, J. Zakel, J. Hernandez, D. Seyfried, and J. Lazar. 2002 . The HC Express Array Kit can provide sensitive and reproducible gene expression detection on DNA microarrays (poster presentation). Biomolecular Technologies: Tools for Discovery in Proteomics and Ge nomics . Austin, TX.

• Obiso, R.J ., S. Bingham, C. Strange, J. Zakel, and D.M. Seyfried. 2002 . Hybrid Capture technology applied to DNA microarrays. Genome Tri - Conference (poster presentation). San Jose, CA.

• R. Obiso . George Washington University, Genomics and Proteomics Interest Group. September 2002 . Gene Expression and Hybrid Capture Technology. Washington, D.C.

• R. Obiso . Virginia Tech, July 2002 . “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, Blacksburg, VA. Oral presentation and Wet - laboratory demonstrations

• R. Obiso . Bio - molecular Technologies: Tools for Discovery in Proteomics and Genomics . March 2002 , “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, Austin, TX.

• R. Obiso . Institute for Systems Biology, March 2002 . “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, Seattle, WA. Oral presentation and Wet - l aboratory demonstrations

• R. Obiso . University of Washington, March 2002 , “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, Seattle, WA Oral presentation and Wet - laboratory demonstrations

• R. Obi so . University of California at San Francisco Gladstone Institute for Cardiovascular Disease, February 2002 , “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, San Francisco, CA.

• R. Obiso . Stanfo rd University, February 2002 , “ A novel non - labeling protocol for the hybridization and direct detection of gene expression on microarrays”, Stanford, CA

• R. Obiso . Ernest Orlando Lawrence Berkley National Laboratory, February 2002 , “ A novel non - labeling p rotocol for the hybridization and direct detection of gene expression on microarrays”, Berkley, CA

• R. Obiso . Cambridge Health - Tech Institute’s Gene Quantification Conference, February 2002 , “Quantitative microarray analysis by hybrid capture”, San Diego CA

• R. Obiso . The Institute for Genomic Research, January 2002 , “Quantitative microarray analysis using hybrid capture”, Rockville, MD.

• R. Obiso . George Mason University, November 2001 , “Bioterrorism and the future of biological threats”, Alexandria, VA.

• R. Obiso. GlaxoSmithKline, October 2001 , “Quantitative microarray analysis using hybrid capture”, Philadelphia, PA

• R. Obiso . Digene Corporation, “Detection of bacteria and their toxins”. July 2001 , Clinical Sciences, Gaithersburg, MD Lazar, J., S. Land ry, C. Strange, J. Zakel, R. Obiso , and D. Seyfried. 2001 . Quantitative microarray analysis by Hybrid Capture (poster presentation) . Chips - to - Hits. San Diego, CA.

• Obiso, R.J . and J. White. 2001 . Electrochemiluminescent detection of bacterial pathogens from environmental surfaces (poster presentation). International Association of Food Protection. Minneapolis, MN

• Obiso, R.J ., and J. White. 2001 . Novel biosensors for the rapid detection of Campylobacter in various food matrices (poster presentation) . International Animal Agriculture and Food Science Conference. Indianapolis, IN

• Obiso, R.J . and J. White. 2001 . Electrochemiluminescent detection of bacterial pathogens from human fecal samples (poster presentation). American Society for Microbiolog y 101 st annual meeting. Orlando, FL.

• Mann, R., M.V. Whitcher, D. Bharti, and R.J. Obiso. 2000 . The role of nutrition and antibiotic administration in the development of intestinal bacterial flora in the premature infant (poster presentation). Biomedica l Engineering Conference, Knoxville, TN.

• Mann, R., M.V. Whitcher, D. Bharti, and R.J. Obiso. 2000 . The role of nutrition and antibiotic administration in the development of intestinal bacterial flora in the premature infant (poster presentation). ETSU Student Research Forum. Johnson City, TN.

• Whitcher, M.V., R. B. Mann, L. Powers, and R. J. Obiso. 2000 . Isolation and characterization of e nterotoxigenic Bacteroides fragilis from e xtraintestinal i solates (poster presentation 138/B). American Society for Microbiology Annual Meeting. Los Angeles, CA.

• Mann, R., M.V. Whitcher, D. Bharti, and R.J. Obiso. 2000. The role of nutrition and antibiotic administration in the development of intestinal bacterial flora in the premature infant (poster presentation). Biomedical Engineering Conference, Knoxville, TN.

• Mann, R., M.V. Whitcher, D. Bharti, and R.J. Obiso. 2000. The role of nutrition and antibiotic administration in the development of intestinal bacterial flora in the premature infant (poster presentatio n). ETSU Student Research Forum. Johnson City, TN.

• Whitcher, M.V., R. Mann, and R.J. Obiso . 1999. Neonatal necrotizing enterocolitis in preterm infants: a nutritional and microbiological approach. Presentation of Independent Study results to the Departm ents of Health Sciences and Applied Human Sciences.

• Whitcher, M.V., R. Mann, R. Joshi, and R.J. Obiso . 1999. Isolation and characterization of enterotoxigenic Bacteroides fragilis from human clinical samples. The Kentucky - Tennessee Regional American Socie ty for Microbiology meeting. Abstract/Poster. Bowling Green, KY

• Mann, R.B., D. Pierre, N. Lizotte, R.J. Obiso, Jr ., and L. Powers. 2000 . Incidence of antibiotic resistance, gelatinase, and cytolysin among Enterococcus faecalis isolated from human clinica l samples and municipal wastewater (poster presentation). American Society for Microbiology General Meeting, Los Angeles, CA.

• R. Obiso . I mperial College, St. Mary’s Campus. “Electrochemiluminescence to detect bacterial pathogens”. September 2000 , MRC P rion Unit, London, UK.

• R. Obiso . IGEN International, “Follow your ‘Gut’ reactions: Intestinal bacterial toxins: mechanism of action”, Industrial Products Group, March 2000 , Gaithersburg, MD

• R. Obiso . Bristol Myers Squibb, “Follow your ‘Gut’ reaction s: Intestinal bacterial toxins: mechanism of action”, Antimicrobial Resistance Research and Development Group. Department of Biological Sciences, March 2000 , Wallingford, CT

• R. Obiso . East Tennessee State University, Microbiology Club, “Careers in Biot echnology”, January 2000 , Johnson City, TN

• R. Obiso , East Tennessee State University, “Bacterial toxins: mechanism of action”, Department of Biological Sciences, January 1999, Jo hnson City, TN

• Walker, E., and R.J. Obiso , Jr. 1999. Localization of the m ature Bacteroides fragilis toxin in membranes (poster presentation). American Society for Microbiology Regional Meeting (Kentucky - Tennessee), Bowling Green, KY.

• Whitcher, M.V., R. Joshi, R. Mann, and R.J. Obiso , Jr. 1999. Isolation and characterizati on of enterotoxigenic Bacteroides fragilis from human clinical samples (poster presentation). American Society for Microbiology Regional Meeting (Kentucky - Tennessee), Bowling Green, KY.

• Mann, R.B., D. Pierre, R.J. Obiso , Jr., and L. Powers. 1999. Inci dence of antibiotic resistance, gelatinase, and cytolysin among Enterococcus faecalis isolated from human clinical samples and municipal wastewater (poster presentation). American Society for Microbiology Region al Meeting (Kentucky - Tennessee), Bowling Gree n, KY.

• R. Obiso , East Tennessee State University, “Bacterial toxins: mechanism of action”, Department of Chemistry, November 1998, Johnson Cit y, TN.

• R. Obiso , East Tennessee State University, Bacteroides fragilis toxin: mechanism of action” , April 19 98, Johnson City, TN.

• R. Obiso , Los Alamos National Laboratory, “A Guide to protein purification”, March 1998, Los Alamos, NM.

• Obiso , R.J., Jr., T.J. Herdendorf, L.A. Vanderberg. 1998. Development of a real - time bacterial endospore viability detection system (poster presentation). 98 th American Society for Microbiology General Meeting. Atlanta, GA.

• Obiso , R.J., Jr. and T.D. Wilkins. The Bacteroides fragilis toxin, fragilysin. In , F. Woessner and A. Barrett (eds.), Handbook of Proteolytic Enzymes (I SBN: 0120793709), Chapter 308, Academic Press Publishing Co., London. – June 1998.

• Obiso , R . Characterization and molecular analysis of fragilysin: the Bacteroides fragilis toxin. Virginia Tech. Ph.D. dissertation. June 1997

• Obiso , R.J., Jr., D. Beva n, and T.D. Wilkins. 1997. Molecular modeling and analysis of the Bacteroides fragilis toxin. Clinical Infectious Diseases. 9(S2): 153 - 155.

• Obiso , R.J., Jr., A.O. Azghani, and T.D. Wilkins. 1997. Bacteroides fragilis enterotoxin disrupts the tight ju nctions of epithelial monolayers. Infection and Immunity. 65(4): 1431 - 1439.

• Lawrence, J.P., L. Brevetti, R.J. Obiso , Jr., T.D. Wilkins, K. Kimura, R. Spoer. 1997. Effects of epidermal growth - factor and Clostridium difficile toxin - B in a model of mucos al injury. Journal of Pediatric Surgery. 32(3): 430 - 433.

• R. Obiso , Los Alamos National Laboratory, “ Bacteroides fragilis toxin: mechanism of action”, April 1997, Los Alamos, NM.

• R. Obiso , California Polytechnic State University, “Anaerobic Diarrheal Pa thogens, March 1997, San Luis Obispo, CA.

• R. Obiso , Kenyon State College, “ Bacteroides fragilis toxin: mechanism of action”, 1997. Kenyon, OH

• R. Obiso , Kenyon State College, “Prokaryotic gene regulation, an overview”, March 1997, Kenyon, OH.

• R. Obiso , Emporia State University, Department of Biology, “Intestinal anaerobic bacteria and the toxins they produce”, February 1997, Emporia, KS.

• Obiso , R.J., Jr., D. Bevan, and T.D. Wilkins. 1996. Topological and sequential relations between the Bacteroides fra gilis enterotoxin with Crotalus adamanteus adamalysin II: the metzincin family of zinc metalloproteinases (oral presentation 3/10). Congress on Anaerobic bacteria and Anaerobic Infections. Chicago, IL.

• Obiso , R.J., Jr., A.O. Azghani, and T.D. Wilkins. 1 996. Bacteroides fragilis enterotoxin perturbs the paracellular barrier function on cultured epithelial monolayers from various cell lines (poster presentation abstracts 205). 96 th American Society for Microbiology General Meeting. New Orleans, LA.

• Bre vetti, L., R.J. Obiso , Jr., T.D. Wilkins, and J.P. Lawrence. Clostridium difficile toxin A: effects on mucosal permeability. (poster presentation abstract 28). 1996 American College of Surgeons Committee on Trauma Symposium. Iowa City, Iowa.

• Brevetti , L., R.J. Obiso , Jr., T.D. Wilkins, and J.P. Lawrence. 1996. The effects of Clostridium difficile Toxin B on mucosal permeability (poster presentation). 1996 American Gastroenterological Association Annual Meeting. Westerville, Ohio.

• Brevetti, L., R.J . Obiso , Jr., T.D. Wilkins, and J.P. Lawrence. 1996. The effect of Clostridium difficile Toxin - B on mucosal permeability. Gastroenterology 110(4): 873 - 874.

• Obiso , R.J., Jr., R.L. Van Tassell, D.M. Lyerly, and T.D. Wilkins. 1995. Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo . Infection and Immunity. 63(10): 3820 - 3826.

• Moncrief, J.S., R.J. Obiso , Jr., L.A. Barroso, J.J. Kling, R.L. Wright, D.M. Lyerly, R.L. Van Tassell, and T.D. Wilki ns. 1995. The enterotoxin from Bacteroides fragilis is a metalloprotease. Infection and Immunity. 63(1): 175 - 181.

• R. Obiso , Virginia Tech, Department of Biochemistry, “What Do I Do After My Undergraduate Degree in Biochemistry?” November 1996 and 1 99 7, Blacksburg, VA.

• R. Obiso , Virginia Tech Phi Sigma Honors Society, “Graduate school – to go or not to go – and, what to expect”. October 1994, 1995, and 1996, Blacksburg, VA.

• Obiso , R.J., Jr., R.L. Van Tassell, D.M. Lyerly, and T.D. Wilkins. 1995. The effects of the Bacteroides fragilis enterotoxin on lamb and rabbit small and large intestine (poster presentation abstracts 185). 95 th American Society for Microbiology General Meeting. Washington, DC.

• Obiso , R.J., Jr. 1995. Bacterial ADP - Ribosyltra nsferases: toxins and related proteins that effect GTP binding proteins (oral presentation). Department of Biochemistry and Anaerobic Microbiology, Virginia Tech.

 

Cited in Acknowledgements

 

• Wells, C.L., E.M.A. Van de Westerlo, R.P. Jechorek, B.A. Feltis, T.D. Wilkins, and S.L. Erlandsen. 996. Bacteroides fragilis enterotoxin modulates epithelial permeability and bacterial internalization by HT - 29 enterocytes. Gastroenterology. 110:1429 - 1437.

• Donelli, G., A. Fabbri, C. Fiorentini. 1996. Bacteroides f ragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT - 29 cells. Infection and Immunity 64(1): 113 - 119.

• Koshy, S.S., M.H. Montrose, and C.L. Sears. 1996. Human intestinal epithelial cells swell and demonstrate actin rearrangeme nt in response to the metalloproteinase toxin of Bacteroides fragilis . Infection and Immunity. 64(11): 5022 - 5028.
 

Research Highlighted in Popular Press

 

• WCYB TV (NBC Affiliate), Karen Cole reporter, Medical Watch Edition: “Study to find cause of Neonatal Ne crotizing Enterocolitis in Premature Infants”. November 1999.

• WJHL TV (CBS Affiliate), Medical Watch Edition: “Researchers at ETSU to find cause of Killer of Premature Infants”. November 1999

• Tri Cities Times News, “Deadly infant disease focus of local study” Headline: ETSU researchers hope to stem disease that kills 4,000 premature babies annually . Published: November 28, 1999.

• WCYB TV (NBC Affiliate), Karen Cole reporter, Medical Watch Edition: “Antibiotic Resistance and what you should know”. Janu ary 2000.

• Going a Label - Free Route with Biosensors. Jun 15 2006 (Vol. 26, No. 12). New Technologies for Calorimetric and SPR Aim to Transcend the Drawbacks to Labels. Written by, Catherine Shaffer

• Genetic Engineering News. Feb 15 2007 (Vol. 27, No.) Lab el - Free Biosensors Show Early Promise. Written by, K. John Morrow, Jr., Ph.D.

• FSNet, Food Safety Network. March 2007. Bacteriophage - based decontamination of Food.