ACE inhibitory peptides with less than 10 KDa were prepared by using natural lactic acid fermented pork as materials in Southwest China. The effects of ACE inhibitory peptide on vascular endothelial cell function were studied by primary culture and subculture of rat thoracic aorta endothelial cells. Result displayed that the content of peptide was 86.54%, and IC50 was 0.895 mg/mL. The hydrophobic amino acids, aromatic amino acids, and branched chain amino acids of the peptides were 39.35%, 10.69%, and 13.65%. The experimental group showed that the cell survival rate in each dose group was above 80%, which indicated that the concentration of each group was suitable. So ACE inhibitory peptides from fermented pork had no obvious toxic effect on vascular endothelial cells in a certain concentration range. These inhibitory peptides could increase the release of NO in endothelial cells and the high dose group was better than the low and medium dose group, but less than the positive control group. Besides, it also could reduce endothelin-1 secretion with the high and medium dose group better than the low dose group but less than the positive control group. No significant in vitro antioxidant capacity and cell reactive oxygen species clearance was observed. The inhibition mechanism of ACE inhibitory peptides from fermented pork may promote vascular endothelial cells to release NO and reduce endothelin-1 secretion.
Fermented pork, ACE inhibitory peptides, Nitric oxide, Endothelin-1, Reactive oxygen species